Journal: Oncology Research

Article Title: Elucidating the Potential Targets and Mechanisms of Bisphenol A-Induced Prostate Cancer Based on Network Toxicology and Molecular Docking Analyses

doi: 10.32604/or.2026.076716

Figure Lengend Snippet: BPA promotes the migration and invasion of PC3 and DU145 cells. ( A ) PC3 and DU145 cells were seeded in the upper chamber of Transwell devices and treated with BPA for 24 h, followed by crystal violet staining of migrated cells. ( B ) Cells were seeded in the upper chamber of Transwell devices coated with Matrigel and allowed to invade for 24 h, after which, the cells were stained and counted. Results are expressed as the means ± SD of three replicate experiments. ** p < 0.01, *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell lines were obtained from Shanghai Model Organisms Company (Shanghai, China; catalog no. NM-H003 and NM-H004).

Techniques: Migration, Staining

Journal: Oncology Research

Article Title: Elucidating the Potential Targets and Mechanisms of Bisphenol A-Induced Prostate Cancer Based on Network Toxicology and Molecular Docking Analyses

doi: 10.32604/or.2026.076716

Figure Lengend Snippet: BPA regulates protein levels of EMT biomarkers and transcription factors in prostate cancer cells. ( A , B ) After 24 h of BPA treatment, the expression levels of N-cadherin, vimentin, MMP2, MMP9, and MT1-MMP were measured in PC3 and DU145 cells. ( C , D ) After 24 h of BPA treatment, the expression levels of Snail, Slug, and Twist1 were measured in PC3 and DU145 cells. Data are shown as the mean ± standard deviation of three independent experiments. ** p < 0.01, *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell lines were obtained from Shanghai Model Organisms Company (Shanghai, China; catalog no. NM-H003 and NM-H004).

Techniques: Expressing, Standard Deviation

Journal: Oncology Research

Article Title: Elucidating the Potential Targets and Mechanisms of Bisphenol A-Induced Prostate Cancer Based on Network Toxicology and Molecular Docking Analyses

doi: 10.32604/or.2026.076716

Figure Lengend Snippet: BPA activates the PI3K/AKT/GSK-3β/β-catenin signaling pathway.( A , B ) BPA enhanced the PI3K/AKT/GSK-3β/β-catenin signaling pathway in a dose-dependent manner; cells were treated with BPA at different concentrations for 24 h. ( C , D ) BPA reversed the effect of the PI3K inhibitor LY294002 on the PI3K/AKT/GSK-3β/β-catenin signaling pathway in prostate cancer cells. Data are shown as the mean ± standard deviation of three independent experiments. ** p < 0.01, *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell lines were obtained from Shanghai Model Organisms Company (Shanghai, China; catalog no. NM-H003 and NM-H004).

Techniques: Standard Deviation

Journal: Oncology Research

Article Title: Elucidating the Potential Targets and Mechanisms of Bisphenol A-Induced Prostate Cancer Based on Network Toxicology and Molecular Docking Analyses

doi: 10.32604/or.2026.076716

Figure Lengend Snippet: BPA promotes the migration and invasion of PC3 and DU145 cells. ( A ) PC3 and DU145 cells were seeded in the upper chamber of Transwell devices and treated with BPA for 24 h, followed by crystal violet staining of migrated cells. ( B ) Cells were seeded in the upper chamber of Transwell devices coated with Matrigel and allowed to invade for 24 h, after which, the cells were stained and counted. Results are expressed as the means ± SD of three replicate experiments. *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell lines were obtained from Shanghai Model Organisms Company (Shanghai, China; catalog no. NM-H003 and NM-H004).

Techniques: Migration, Staining

Journal: Biochemistry and Biophysics Reports

Article Title: Investigating the ABTS-based antioxidant potential and antiproliferative activity of pulp, skin and seeds extracts from Nephelium lappaceum , Manilkara zapota , and Meliccocus oliviformis on human prostate and colon cancer cells

doi: 10.1016/j.bbrep.2025.102257

Figure Lengend Snippet: Effects of the different fruit extracts on the proliferation of the colon cancer cell line HCT116 and the prostate cancer cell line DU145 . DU145 (A) or HCT116 (B) were seeded in a 12-well plate and allowed to grow for 12 h. Then, 500 μg/mLof PBS-resuspended extract derived from the pulp, seeds, and skin of Meliccocus oliviformis , Nephelium lappaceum , and Manilkara zapota were added and cell proliferation was monitored by analyzing the surface area occupied by the cells in each well (% confluence) from photos taken at regular intervals (every 3 h) by the Incucyte live cell imaging system over a period of three days. Data shown are the average ± SD of three independent experiments.

Article Snippet: The HCT116 (colic), and DU145 (prostatic) human cancer cell lines were purchased from ATCC (American Type Culture Collection).

Techniques: Derivative Assay, Live Cell Imaging

Journal: Biochemistry and Biophysics Reports

Article Title: Investigating the ABTS-based antioxidant potential and antiproliferative activity of pulp, skin and seeds extracts from Nephelium lappaceum , Manilkara zapota , and Meliccocus oliviformis on human prostate and colon cancer cells

doi: 10.1016/j.bbrep.2025.102257

Figure Lengend Snippet: Effects of the different fruit extracts on the viability of the colon cancer cell line HCT116 and the prostate cancer cell line DU145 . DU145 (A) or HCT116 (B) were seeded in a 12-well plate and allowed to grow for 12 h. Then, 500 μg/mL of PBS-resuspended extract derived from the pulp, seeds, and skin of Meliccocus oliviformis , Nephelium lappaceum , and Manilkara zapota were added. 24h later, cell viability was assessed using the MTT assay. Data shown are the average ± SD of three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, one-way ANOVA followed by Tukey's multiple comparison test.

Article Snippet: The HCT116 (colic), and DU145 (prostatic) human cancer cell lines were purchased from ATCC (American Type Culture Collection).

Techniques: Derivative Assay, MTT Assay, Comparison